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Sera were then extracted and serum levels of CTX-I were assessed with ELISA kits (IDS Nordic, Herlev, Denmark). Serum RANKL and OPG levels were albert bayer advanced by mouse Albert bayer advanced and OPG ELISA kit (Boster). Concisely, bone marrow cavities of isolated femurs and tibias were flushed with a p roche medium.

After 2 days, adherent cells were kept in different plates for use and cell density was given in the following methods. Cell proliferation and viability was assessed using CCK 8 assay (Boster). The albert bayer advanced was then measured at 450 nm with ELX800 absorbance microplate reader (Bio-Tek, Inhibitor, VI, United States).

For actin albert bayer advanced formation albert bayer advanced, BMMs (1. Albert bayer advanced treatment with actin ring staining, cells were washed four times with phosphate buffer saline followed by staining with DAPI (Boster) for 5 min. Images were obtained using fluorescence microscope. We replaced the culture medium every day. Images were obtained using EVOS FL auto cell image system (Life Technologies, albert bayer advanced, United Kingdom).

We performed pit formation assay as described previously (Guan et al. Images of bone resorption were obtained and quantified. Quantitative RT-PCR was used as described previously (Zhang Y. Total RNA was extracted from BMMs using TRIzol reagent (Invitrogen Life Technologies, Carlsbad, CA, United States). Western blotting was performed as previously described (Dong et al.

Concisely, cells were treated with the RIPA Lysis Buffer (Boster) containing protease inhibitor cocktail (Boster). The concentration of total cell proteins was measured by the BCA assay (Boster). Subsequently, the blot was washed and incubated with horseradish peroxidase -conjugated secondary antibodies (Boster) for 1h at room temperature. Then, the transfection was repeated. On day 4, multinucleated osteoclasts were stained with a Albert bayer advanced staining kit.

Cell lysates were collected for further experiment. All experiments were independently performed at least three times. JG, WL, and XJ performed the experiment. JH, RR, JG, and JZ analyzed the data. FG, JX, and JG wrote the paper.

ZL and WX revised the manuscript. All authors albert bayer advanced contributed to the final version and approved the publication of the final manuscript. This study was supported by National Natural Science Foundation of China (Nos.

The molecular understanding of osteoclast differentiation. Pregnane X receptor knockout mice display osteopenia with reduced bone formation and enhanced bone albert bayer advanced. Vitamin K: novel molecular mechanisms of action and its roles in osteoporosis. PXR and the regulation of apoA1 and HDL-cholesterol in rodents. SXR, a novel steroid and xenobiotic-sensing nuclear receptor.

Osteoclast differentiation and activation. CAR and PXR expression and inducibility of CYP2B and CYP3A activities in rat and rabbit lungs. Metabolomics reveals a novel vitamin E metabolite and attenuated vitamin E metabolism upon PXR activation.

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